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KMID : 1094720000050060413
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 6 p.413 ~ p.417
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Direct triazine herbicide detection using a self-assembled photosynthetic reaction center from purple bacterium
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Nakamura Chikashi
Hasegawa Miki
Shimada Kazumi
Shirai Makoto
Miyake Jun
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Abstract
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In this study, a direct detection system for triazine derivative herbicides was developed using the photosynthetic reaction center (RC) from the purple bacterium,Rhodobacter sphaeroides, and surface plasmon resonance (SPR) apparatus. The histidine-tagged RCs were immobilized on an SPR gold chip using nickel-nitrilotriacetic acid groups as a binder for one of the triazine herbicide, atrazine. The SPR responses were proportional to the sample concentrations of atrazine in the range 0.1?1 μg/mL. The sensitivity of the direct detection of atrazine using the RC-assembled sensor chip was higher than that using the antibody-immobilized chip. The other types of herbicides, DCMU or MCPP, were not detected with such high sensitivity. The results indicated the high binding selectivity of the RC complex.
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KEYWORD
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photosynthetic reaction center, herbicide, triazine derivative, surface plasmon resonance, biosensor, affinity binding
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